Ultrasonic kev cuam tshuam yog cov khoom tseem ceeb hauv cov khoom siv biotechnology niaj hnub no. Nws tuaj yeem ua tiav cov haujlwm xws li kev cuam tshuam ntawm tes, cov khoom homogenization thiab nanomaterial dispersion dhau ntawm cavitation cov nyhuv tau tsim los ntawm cov siab ntev. Cov kab lus no qhia txog tus ua haujlwm ua haujlwm, cov qauv kev thov ntawm cov ntawv thov kev lag luam ntawm kev tshawb fawb thiab siv cov khoom siv no.
1. Ua haujlwm ua haujlwm
1.1 Tub Ntxhais Kawm Lub Cev
Ultrasonic kev cuam tshuam tsuas yog cia siab rau cov cavitation ua kom ua tiav kev cuam tshuam ntawm tes:
Lub zog hloov pauv lub zog hluav taws xob rau hauv siab - zaus tshuab hluav taws xob deeg ntawm 20kHz - 1mHz
Ultrasonic tsis propagate nyob rau hauv cov kua media, tsim kev hloov siab siab - siab thiab qis - Kev Nyuaj Siab
Lub tshuab nqus tsev me me (cavitation nuclei) tau tsim nyob rau hauv qhov tsawg - Siab theem
Npuas Chais tawm tsam cov neeg siab siab nyob rau hauv lub siab siab - theem siab, tsim kev kub siab hauv zos siab (txog 5000atm) thiab muaj zog poob siab vuag
2. Cov khoom lag luam yooj yim ntawm lub ntsuas
Niaj hnub no ultrasonic cell cuam tshuam feem ntau muaj cov hauv qab no:
Lub tshuab hluav taws xob: muab cov cim hluav taws xob siab (feem ntau 20 - 40kHz)
Transducer: Piezoelectric Ceramic Muaj Cov Hluav Taws Xob Tshawb Xyuas Hluav Taws Xob - Tshuab Kho Tshuab Zog
Amplifier: titanium alloy probe, Amplifies Kev Txhim Kho Cov Khoom Muag
Cua Txias Txheej Txheem: Tiv thaiv cov qauv los ntawm kev kub heev (yeem)
Chav Tswj Tswj: Hloov lub zog, sijhawm, lub sijhawm mem tes
3. Daim ntawv thov teb
3.1 Kev Tshawb Fawb Lub Neej Kev Tshawb Fawb
Protein Extraction: Escherichia Coli suav lub cev xaus kev ua haujlwm> 90%
DNA / RNA npaj: Siv ua ke nrog cov khoom siv kom nce tawm los ntawm 30 - 50%
Subcellular Cheebtsam sib cais: Ua tiav cov kev rho tawm ntawm cov koom ua ke xws li Mitochondria thiab Chloroplas thiab Chloroplas
Cov kab mob hloov pauv: Txhim kho kev ua haujlwm ntawm kev ua yeeb yam DNA exna
3.2 nanomaterials teb
Nanoparticle dispersion: tuaj yeem txo cov particle loj ntawm <100nm
Graphene exfoliation: kua theem ultrasound los npaj ib leeg - Txheej graphene
Nanoemulsion Kev Npaj: Tau noanemulsion nrog uniform particle loj faib
3.3 Kev Siv Cov Ntawv Thov
Khoom noj khoom haus: Kua txiv Homogenization, poov xab cell phab ntsa kov
Biofule: microalgae cell cuam tshuam kev cog qib los txhim kho cov nqaij lipid extraction
Kev Kho Mob Dej Sov: Txhim kho sludge anaerobic zom ua haujlwm
Ultrasonic qhov kev cuam tshuam ntawm tes muaj kev cuam tshuam tshwj xeeb hauv kev tswj hwm cov haujlwm ntawm biomolecules vim nws lub cev ua haujlwm thiab tsis yog - hu ua cov yam ntxwv. Nrog rau kev txhim kho cov tshuaj kho mob thiab cov khoom siv Nanomaterials, cov khoom siv ua ultrasonic yuav dhau los ua cov qauv kev ua lag luam roj ntsha thiab cov kab ntau lawm. Kev tshawb fawb yav tom ntej yuav tsum tsom rau kev hloov kho lub zog hloov kev ua haujlwm thiab tsim cov kev daws teeb meem rau cov qauv ua tiav.
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Dab tsi yog ultrasonic cell cuam tshuam?
Cov txheej txheem