Lub hauv paus ntsiab lus ntawm Ultrasonic Extraction
Ultrasonic tsis propagate los ntawm cov xov xwm tawm, ua cov "cavitation nyhuv" thaum lub sijhawm kev co. Cov ntsiab lus cuam tshuam cov qauv siab ua haujlwm siab - Zog cov microbebles, uas tso tawm hauv zos kub siab thiab qhov siab siab thaum lawv tawg, yog li:
1.Destroying xovtooj ntawm tes: Txhawb kev tawm ntawm cov txheej txheem intracellular.
2.Txoj kev tiv thaiv thaj chaw tiv tauj ntawm cov kuab tshuaj thiab cov qauv: nce kev sib cais thiab diffusion tus nqi.
3.Txoj kev hloov pauv loj: nrawm hloov cov khoom xyaw nquag los ntawm cov qauv rau cov kuab tshuaj.
Ultrasonic rho tawm cov kauj ruam
1. Npaj raw khoom siv:
Zuaj lub hom phiaj khoom (xws li cov nroj tsuag lossis lwm yam qauv khoom) kom nce thaj tsam thaj tsam.
Xaiv cov kuab tshuaj uas tsim nyog (xws li dej, ethanol, methanol, methanol lossis lwm yam polar daws) raws li xav tau.
2. Hnyav Sib xyaw:
Sib tov cov qauv thiab cov kuab tshuaj nyob rau hauv ib qho kev sib piv uas tsim nyog, feem ntau 1: 5 txog 1:20 (qauv: tshuaj tiv thaiv).
Tso qhov sib tov sib xyaw hauv ib lub taub ntim tiv taus rau ultrasonic kev co.
3. Ultrasonic kev kho mob:
Siv kev sojntsuam ultrasonic lossis ultrasonic nqus tsuag.
Teeb lub zaus thiab lub hwj chim (nquag nquag yog 20-40 KHz) thiab tswj qhov kub ntawm 30-70 ° C.
Lub sijhawm rho tawm feem ntau yog 10-60 feeb, uas tuaj yeem hloov kho nyob ntawm cov qauv thiab hom phiaj.
4. Sib cais thiab ua kom huv:
Siv centrifugation, pom kev lossis ib qho kev sib cais funnel cais cov tshuaj los ntawm cov khoom.
Mloog zoo, qhuav lossis ntxiv purify cov extract (xws li kem chromatography).
