Umgaqo we-ultrasonic yokukhupha
Amaza e-ultrasonic avelisa imithombo yeendaba engamanzi, evelisa "isiphumo" se-cavite "ngexesha lokuhluza. Impembelelo yeqhinga le-cavite - ii-microbubble zamandla, ezikhupha amaqondo obushushu asekhaya kunye noxinzelelo oluphezulu xa ziqhekeza, ngalo:
1.Detharethariting yeseli: Ukukhuthaza ukukhutshwa kwezinto ze-intracellillar.
2.Umda woqhakamshelwano phakathi kwesinyibilikisi kunye nesampulu: ukwandisa ukutshitshiswa kunye nenqanaba lokupheliswa.
3.Ndinyanzele inkqubo yokudluliselwa kwesininzi: Ukukhawulezisa ukudluliselwa kwezithako ezisebenzayo ukusuka kwisampulu ukuya kwisinyibilikisi.
Amanyathelo okukhutshelwa kwe-Ultrasonic
1. Lungisa izinto ezingafunekiyo:
Kutyumshele izinto ekujoliswe kuzo (ezinjengezityalo okanye ezinye iisampulu eziqinileyo) ukonyusa indawo yomphezulu.
Khetha izinyibilikisi ezifanelekileyo (ezinjengamanzi, ethanol, i-methanol okanye ezinye izinyibilikisi) njengoko kufuneka.
2. Ukuxuba kokucombulula:
Hlanganisa isampulu kunye nesinyibilikisi kumyinge ofanelekileyo, dlanga 1: 5 kuye ku-1: 20 (isampulu) ye-Solvent).
Beka umxube kwisitya esinganyangekiyo kwi-ultrasonic vibration.
I-3. Unyango lwe-Ultrasonic:
Sebenzisa iProsossonic Probe okanye isicoci se-ultrasonic.
Seta i-frequency kunye namandla (amandla aqhelekileyo angama-20 ukuya kwengama-20 khz) kwaye ulawule ubushushu kwi-30-70 ° C.
Ixesha lokukhupha ngokubanzi yimizuzu eli-10-60, elinokulungiswa kuxhomekeka kwisampulu kunye nezinto ekujoliswe kuzo.
4. Ukwahlula kunye nokucocwa:
Sebenzisa i-Greatrifugation, ukuvisa okanye i-funnel ye-funnel yokwahlula isisombululo kwisimo esiqinileyo.
Gxila, womile okanye uqiniseke ngakumbi isicatshulwa (esifana nekholamu chromatography).
