Molao-motheo oa Ts'ebetso ea UltrasonIC
Maqhubu a ultrasonic a phatlalatsa mecha ea litaba ea metsi, a hlahisa "phello ea likhomo" nakong ea vibrate. Phello ea cavitation e na le e phahameng - e nang le litheko tsa boleng bo phahameng ba thepa le likhatello tse phahameng ha li phatloha, ka tsela eo:
1. Marako a CelDrong Cell: Ho khothaletsa ho lokolloa ha likarolo tsa intraclulung.
2.Increage, sebaka sa ho ikopanya pakeng tsa solvent le sampole: Ho eketsa dissulution le sekhahla se fapaneng.
3. Anner ts'ebetso ea phetisetso e kholo: ho potlakisa phetiso ea metsoako e sebetsang ho tsoa ho sampole ho isa bollo.
Mehato ea ho ntša Mehato ea Ultrasoc
1. Lokisetsa lisebelisoa tse tala:
Fokotsa thepa ea sepheo (joalo ka limela kapa disampole tse ling tse tiileng) ho eketsa sebaka se ka holimo.
Khetha li-solvent tse loketseng (joalo ka metsi, ethano, methanol kapa li-solvent tse ling tsa polar. Ha ho hlokahala.
2. Soliven ho kopanya:
Kopanya sampole le solvent ka tekanyo e nepahetseng, hangata 1: 5 ho 1: 5 ho 1:20 (mohlala: solvent).
Beha motsoako ka sejaneng se hanyetsanang le moferefere oa ultrasonic.
3. Phekolo ea ultrasonic:
Sebelisa probe ea ultrasoc kapa ho hloella ha ultrasonic.
Beha khafetsa le matla (maqhubu a tloaelehileng a 20-40 k.0) le ho laola mocheso ka 30-70 ° C.
Nako ea ho tlosa hangata ke metsotso e 10-60 ka kakaretso, e ka fetoloang ho latela mohlala le likarolo tse peli.
4. Karohano le tlhoekiso:
Sebelisa Centrifugation, filimi kapa kemiso e arohaneng ea ho arola tharollo e tsoang ho tse tiileng.
Tsepamisa maikutlo, o omelletse kapa o hloekise konopo (joalo ka kholomo ea clumm marctor).
