Yuav siv cov khoom siv ultrasonic homogenization rau nceb extraction?

Ultrasound - Pab extraction (UAAE) yog cov thev naus laus zis uas siv ultrasonic cavitation cov nyhit rau cov khoom xyaw muaj txiaj ntsig los ntawm cov nroj tsuag. Nws yog qhov tshwj xeeb tshaj yog tsim nyog rau kev rho tawm ntawm polysaccharides, phenols, triterpenes thiab lwm yam khoom xyaw ua kom nquag plias hauv nceb. Hauv qab no yog nws cov txheej txheem thiab cov ntsiab lus tseem ceeb:

1. Cov Khoom Siv Raw Ua Ntej
Ntxuav thiab ziab: cov nceb tshiab yog ntxuav thiab hlais rau hauv daim, feem ntau yog khov (los khaws cov khoom xyaw nquag) lossis cov nqi kub).
Crushing thiab sieving: Tom qab ziab, tsoo rau 40 - 80 mesh (particle loj li ntawm 0.2 - 0.5 hli) kom nce thaj chaw tiv tauj.

2. Ultrasonic rho tawm cov kauj ruam
Kev Xaiv Cov Khoom Siv: Kev sojntsuam ultrasonic Ntsuas (kev sib cuag dua tshiab) lossis Tank Batch (loj pob), qhov zaus feem ntau yog 20 - 40kHz.

Kev xaiv cov kuab:
Dej: Haum rau polysaccharides (xws li Lentinan), Kub 60 - 80 - 80 - 80.
Organic cov kuab tshuaj: ethanol (50 - 70%) yog siv rau phenols thiab triterteres (xws li kev xav tau kom tsis txhob muaj protein denaturation.
Acid - Roog Pab: Dilute acid / paus tuaj yeem rhuav tshem cov phab ntsa xov tooj (xws li chitin) thiab txhim kho cov chitin) thiab txhim kho cov chaitin) thiab txhim kho cov chitrein) thiab txhim kho cov chitrein) thiab txhim kho cov chitrein) thiab txhim kho cov chitrein) thiab txhim kho cov chitrein) thiab txhim kho cov chitical.

Parameter Optimization:
Lub Hwj Chim: 100,700 (ntau dhau yuav ua rau kev ua phem).
Sijhawm: 10 - 60 feeb (dhau lawm ntev yuav ua rau oxidation).
Qhov kub thiab txias: Feem ntau tswj tau ntawm 50 - 70 ℃ (qhov kub (siab (siab dua yuav tsum tau hws thiab ua kom tsis txhob muaj kev loj hlob).

3. Ncej - Kev Kho Mob
Khoom - Cov kua ua kua sib cais: Centrifugation (4000 - 8000rpm, 10 feeb) lossis pom kev me me (0.45μm membrane).
Cov siab thiab ua kom huv si: teb av noo endration convapration, ntxiv kom huv ntawm cov hom phiaj los ntawm macroporous resin, lim ntshav lossis kem chromatography.
Kom qhuav

4. Qhov zoo thiab ceev faj
Qhov Zoo:
Cov sijhawm rho tawm yog luv los ntawm 50 - 70%, thiab cov kev siv daws tau yog txo los ntawm 20 - 50%.
Polysaccharide tawm tuaj yeem nce los ntawm 15 - 30% (piv nrog cov dej ntws ib txwm rho tawm).

Cov kev ceev faj:
Ultrasound tej zaum yuav tsim cov oxygen hom (Ros), thiab cov tshuaj tiv thaiv (xws li 0.1% vitamin C) yuav tsum tau ntxiv.
Qee qhov sib xyaw (xws li cov protein) yuav raug kho vim yog cavitation teebmeem, thiab cov tsis xav tau ua kom zoo.

Daim Ntawv Thov Piv Txwv: Lentinan Polysaccharide Extraction
Cov Zej Zog: Dej raws li cov kuab tshuaj, khoom - kua piv 1:30, 60 ℃ ultrasonic rau 40 feeb (300w).
Cov txiaj ntsig: polysaccharid tawm tuaj yeem ncav cuag 8 - 12%, tseem ceeb dua 5 - 7% ntawm cov txheej txheem ib txwm muaj.