Ungayisebenzisa kanjani imishini ye-homogenization ye-ultrasonic ye-mushroom extraction?

I-Ultrasound - Ukukhishwa Okusizwayo (i-UAE) ubuchwepheshe obusebenzisa umphumela we-ultrasonic cavitation ukuthola kahle izithako ezisebenzayo ezitshalweni. Ilungele ikakhulukazi ukukhishwa kwama-polysaccharides, ama-phenols, ama-triiterpenes nezinye izithako ezisebenzayo kumakhowe. Okulandelayo inqubo yayo ejwayelekile namaphuzu asemqoka:

1
Ukugeza nokomisa: amakhowe amasha ahlanzwa futhi asikiwe noma anciphise abe yizicucu, kuvame ukugcwala - omisiwe (ukugcina izithako ezisebenzayo) noma umoya oshisayo omile (izindleko eziphansi).
Ukuchoboza nokuvinjelwa: Ngemuva kokumiswa, crush to 40 80 mesh (usayizi wezinhlayiyana ezingaba ngu-0.2 - 0.5mm) ukwandisa indawo yokuxhumana.

2. Izinyathelo Zokukhishwa Kwe-Ultrasonic
Ukukhetha imishini: Sebenzisa insimbi ye-Ultrasonic (ukuxhumana ngqo, ukuqina okuphezulu) noma ithuluzi lethangi le-ultrasonic (ukwelashwa okungaqondile, imvamisa enkulu kuvame ukuba ngu-20 - 40Khz.

Ukukhetha kwe-solvent:
Amanzi: Ilungele ama-polysaccharides (anjengeLentinan), izinga lokushisa 60 - 80 ℃.
I-Organic Solvent: I-Ethanol (50 - 70%) isetshenziselwa ama-Phenols kanye nama-triterpenenes (njenge-ganodic acid), kanye nokuhlushwa kudinga ukulawulwa ukuze kugwenywe ukungcola kwe-protein.
I-acid - isisekelo sosizo: I-Diliful acid / base Ungabhubhisa izindonga zamaseli (njenge-chitin) futhi uthuthukise isilinganiso sokukhishwa.

Ukusebenziseka kwepharamitha:
Amandla: 100 - 500W (Kuphezulu kakhulu kungadala ukonakala).
Isikhathi: 10 - Imizuzu engama-60 (isikhathi eside kakhulu ingadala i-oxidation).
Ukushisa: Imvamisa elawulwa ngo-50 - 70 ℃ (Amazinga okushisa aphezulu adinga ukuguqulwa kanye nokuboniswa ukuvikela ukuguquguquka).

3. Okuthunyelwe - Ukwelashwa
Kuqinile - Ukuhlukaniswa okuwuketshezi: Centrifugation (4000 - I-8000rpm, imizuzu eyi-10) noma i-filtration (0.45μm membrane).
Ukugxila kanye nokuhlanza: Ukuhlushwa okujikelezayo kwe-Rotary, Ukuhlanzwa okuqhubekayo kwezinto eziqondisiwe yi-Resin ye-Macroporous, i-Dialysis noma ikholomu ye-chromatography.
Ukomisa: Ukumisa ukomisa (ukushisa - Izakhi ezibucayi) noma zomiswa zokufafaza (ezinkulu - ukukhiqizwa kwesikali).

4. Izinzuzo kanye nokuqapha
Izinzuzo:
Isikhathi sokukhishwa sifinyezwa ngo-50 - 70%, futhi ukusetshenziswa kwe-solvent kuncishiswa ngo-20 - 50%.
Isivuno se-polysaccharide singandiswa ngo-15 - 30% (ngokuqhathaniswa nokukhishwa kwamanzi okushisayo kwendabuko).

Izinyathelo zokuzivikela:
I-Ultrasound ingakhipha izinhlobo ze-oxygen ezisebenzayo (ROS), kanye nama-antioxidants (njenge-0.1% vithamini C) kudingeka ukuthi yengezwe.
Ezinye izakhi (ezinjengamaprotheni) zingabonakala ngenxa yemiphumela ye-cavitation, futhi amapharamitha adinga ukulungiswa.

Isibonelo sesicelo: Ukukhishwa kweLentinan PolysacCharide
Imibandela: Amanzi njenge-solvent, eqinile - isilinganiso se-liquid 1:30, 60 ℃ Ultrasonic imizuzu engama-40 (300W).
Imiphumela: Isivuno se-polysaccharide singafinyelela ku-8 - 12%, siphakeme kakhulu kunale 5 - 7% yendlela yendabuko.